International Journal of Urology Sciences

International Journal of Urology Sciences


International Journal of Urology Sciences
International Journal of Urology Sciences
2021, Vol. 3, Issue 1
Characterization and antibiotic susceptibility profile of Escherichia coli from semen of male patients with infertility

Lurwan Mu’azu, Muhammad S Abdallah, Mustapha Sani3, Nasiru S Gital, Muhammad Ali

The study was aimed to characterize and determine the antibiotic sensitivity pattern of Escherichia coli isolated from semen of male patients with infertility attending Murtala Muhammad Specialist Hospital (MMSH) Kano, Nigeria. One hundred and sixty five (165) Semen specimens were collected from males with infertility attending the clinic and General out Patient Department of MMSH Kano. The seminal fluids were diluted with sterile saline, centrifuged and cultured on Nutrient agar, Blood agar, Chocolate agar and MacConkey agar then incubated aerobically and in 5% CO2 at 37°C for 24 hours for the isolation of pathogenic microorganism. Isolates were identified based on Gram’s staining, biochemical tests and API 20E Test. The result shows a total of seventy six (76) isolates were obtained, Staphylococcus aureus was found to have the highest occurrence of 31 (40.79%), whereas the least was found to be Mycoplasma species, 1 (1.32%). Other microorganisms encountered include; Coagulase negative Staphylococcus species 14 (18.42%), Escherichia coli 11 (14.47%), Klebsiella pneumonia 6 (7.89%), Proteus mirabilis 5(6.58%), Pseudomonas aeruginosa 6 (7.90%) and Neisseria gonorrhoeae 2 (2.63%). The sensitivity and resistivity of E. coli against the antibiotics, the result showed high sensitivity of isolates to ciprofloxacin (81.8%), ofloxacin (72.7%), co-trimoxazole (63.3%) and gentamicin and augmentin ((54.5%) each. On the other hand, high degree of resistivity rates to tetracycline (81.8%), Cefuroxime (63.6%) and ceftriaxone (54.5%), were detected. It has been concluded that E. coli is one of the organism associated with infertility in men.
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How to cite this article:
Lurwan Mu’azu, Muhammad S Abdallah, Mustapha Sani3, Nasiru S Gital, Muhammad Ali. Characterization and antibiotic susceptibility profile of Escherichia coli from semen of male patients with infertility. International Journal of Urology Sciences, Volume 3, Issue 1, 2021, Pages 01-05
International Journal of Urology Sciences International Journal of Urology Sciences